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Cardiology

Project Leader

Jason T. Maynes, MD, PhD
Hospital for Sick Children, Toronto
Adjunct Associate Professor of Biochemistry, University of Toronto
Wasser Chair, Anesthesia and Pain Medicine
Director of Research, Anesthesia and Pain Medicine
Associate Chief of Perioperative Services, Research Perioperative Services
Research Description:
The research in Dr. Mayne's lab focuses on three main areas that attempt to continue my clinical training in paediatrics and anaesthesia with my research training in biophysics: (1) the mechanism of anaesthetic action and anaesthetic off-targets, (2) proteins involved in mitochondrial dynamics and, (3) high-content imaging and image analysis.
To study mitochondria and other cellular processes, Dr. Maynes employs high-content imaging. Unlike traditional confocal microscopy, we image thousands of cells at a time using 96-or 384-well plates and use automated image processing algorithms to determine specified metrics from every cell in every image. This removes the traditional bias associated with manual characterization of confocal images. He has developed machine learning algorithms that automatically identify, quantify, and then classify mitochondrial shape and function in a cell population of interest. He also extended these techniques to other disease phenotypes of relevance to paediatric anaesthestia including pulmonary stenosis, dilated cardiomyopathy and chemotherapy-induced cardiac dysfunction. Using custom assays, we are able to perform high-throughput drug screening to look for agents that attenuate or eliminate the disease phenotype observed.

Members

John Coles, MD, FRCSC
Hospital for Sick Children, Toronto
Senior Associate Scientist, Translational Medicine
Professor, Department of Surgery at University of Toronto
Hospital Tissue Bank, Medical Co-Director
Research Description:
Dr. John Coles received his medical degree from the University of Western Ontario, and his FRCSC in Cardiovascular and Thoracic Surgery in 1982. He has had internships and residencies in Vancouver, London, and Toronto. Dr. Coles joined the SickKids team in 1984 as a consulting staff surgeon, taking on a position in the Research Institute in 1990. He also works in the academic community as a professor in the Department of Surgery at the University of Toronto.
His research studies are directed towards understanding the molecular biology of hypertrophy which occurs in congenital heart disease. He specifically focuses on signaling cascades which promote beneficial compensatory cardiac hypertrophy. His lab is also focused on the characterization of cardiac-progenitor cells resident within the heart in congenital heart disease. Both areas of study involve mouse models of cardiac hypertrophy and parallel analysis of clinical samples exhibiting various degrees and types of hypertrophy.
Craig Simmons, BSc, SM, Phd, FCSME, PEng
Distinguished Professor of Mechanobiology & Interim Director
 
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Joseph Arnold Fisher M.D., FRCP(C)

Professor, Faculty of Medicine, University of Toronto, Canada

 

Dr. Fisher is a Professor, Faculty of Medicine, University of Toronto, Canada, and Senior Scientist, Division of Advanced Diagnostics, Cardiovascular at Toronto General Research Institute, Toronto, Canad. He is the Director of Thornhill Research Inc, a for profit, self-sustaining, spin-off affiliated company from the University Health Network tasked with developing, prototyping, and commercializing health products arising out of original research performed by Dr. Fisher, his colleagues and students. All products are made available for research and clinical use without restriction subject only to ethical and regulatory review. Dr. Fisher has over 30 patents including 12 pending and has been awarded the 2015 Innovation Awards awarded by Innovation Fund Provincial Oversight Committee (IFPOC).National Research Council (Canada) Research award J.A. Fisher.